macsprep chimerism cd19 microbeads human kit (Miltenyi Biotec)
Structured Review

Macsprep Chimerism Cd19 Microbeads Human Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/macsprep chimerism cd19 microbeads human kit/product/Miltenyi Biotec
Average 92 stars, based on 2 article reviews
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1) Product Images from "High purity high yield tandem B and T helper cell isolation for qRT-PCR analysis suitable for basically equipped laboratories"
Article Title: High purity high yield tandem B and T helper cell isolation for qRT-PCR analysis suitable for basically equipped laboratories
Journal: Malaria Journal
doi: 10.1186/s12936-018-2547-3
Figure Legend Snippet: List of fluorophore-conjugated antibodies
Techniques Used:
Figure Legend Snippet: Flow cytometry analysis of purity and efficiency of B and Th cell isolation. Shown is a representative analysis of three independent isolations. a Flow cytometry analysis of whole blood after erythrocyte lysis (= before B cell isolation). Upper left: gating of live lymphocytes. Lower left: gating of single cells. Upper right: total T cells (CD3+) versus B cells (CD19+). Lower left: total T cells (CD3+) versus Th cells (CD3+CD4+). b Flow cytometry analysis of isolated CD19+ B cells (= positive fraction after B cell isolation). Upper left: gating of live lymphocytes. Lower left: gating of single cells. Upper middle: total T cells (CD3+) versus B cells (CD19+). Lower middle: total T cells (CD3+) versus Th cells (CD3+CD4+). Upper right: histogram of CD19 positive cells. Lower right: histogram of CD4 positive cells. c Flow cytometry analysis of isolated CD4+ Th cells (= positive fraction after Th cell isolation). Upper left: gating of live lymphocytes. Lower left: gating of single cells. Upper middle: total T cells (CD3+) versus B cells (CD19+). Lower middle: total T cells (CD3+) versus Th cells (CD3+CD4+). Upper right: histogram of CD19 positive cells. Lower right: histogram of CD4 positive cells. d Flow cytometry analysis of the negative fraction after Th cell isolation. Upper left: gating of live lymphocytes. Lower left: gating of single cells. Upper middle: total T cells (CD3+) versus B cells (CD19+). Lower middle: total T cells (CD3+) versus Th cells (CD3+CD4+). Upper right: histogram of CD19 positive cells. Lower right: histogram of CD4 positive cells
Techniques Used: Flow Cytometry, Cell Isolation, Lysis, Isolation